Method for improving turfgrass abiotic stress tolerance

ABSTRACT

The present invention relates to a method of controlling abiotic stress on warm-season turfgrass using an effective amount of acibenzolar-s-methyl.

RELATED APPLICATION INFORMATION

This application is a continuation of Ser. No. 15/523,850 filed 2 May2017, which is a 371 of International Application PCT No.PCT/US2015/058288, filed 30 Oct. 2015, which claims the benefit of U.S.Provisional Application No. 62/074,114, filed 3 Nov. 2014, the contentsof which are incorporated herein by reference herein.

FIELD OF TECHNOLOGY

The present invention relates to methods of improving the abiotic stresstolerance of turfgrass. More specifically, the present invention relatesto methods of improving the abiotic stress tolerance of warm-seasonturfgrass with acibenzolar-s-methyl and, optionally, trinexapac-ethyl.

BACKGROUND

There are numerous problems that turfgrass managers face in maintainingturfgrass at a standard of quality expected by users. While the problemsare many, those relating to abiotic stress (including drought stress)are particularly challenging to manage and control. For example, abioticstress can affect turfgrass plants on golf courses causing a loss ofrevenue from reduced playability. One example of a common problem forgolf course managers is abiotic stress caused by drought or heat.

The compound acibenzolar-S-methyl (S-methylbenzo[1,2,3]thiadiazole-7-carbothioate) acts as a functional analogue ofthe natural signal molecule for systemic activated resistance (SAR),salicylic acid. It activates the host plant's natural defence mechanism.The structure of acibenzolar-S-methyl can be represented as follows:

Acibenzolar-S-methyl is commercially available under the trade nameActigard® (Syngenta Crop Protection, Inc.).

Plant growth regulators are often used to regulate the growth anddevelopment of turfgrasses as well as crops. There are several differentclasses of plant growth regulators. Known classes include azoles (suchas uniconazole, and paclobutrazol), cyclohexane carboxylates (such astrinexapac-ethyl, and prohexadione-calcium), pyrimidinyl carbinols (suchas flurprimidol, and ancymidol), quarternary ammoniums (such aschlormequat-chloride, and mepiquat-chloride), and sulphonyl-aminophenyl-acetamides (such as mefluidide).

Plant growth regulators operate by various modes of action. For example,onium-type plant growth retardants such as chlormequat-chloride andmepiquat-chloride, that possess a positively charged ammonium,phosphonium or sulphonium group, function by blocking the synthesis ofgibberellin early in the biosynthetic pathway. Growth retardantscomprising a nitrogen-containing heterocycle, such as flurprimidol,paclobutrazol and uniconazole-P, act as inhibitors of monooxygenasesthat catalyse oxidative steps in gibberellin biosynthesis. Structuralmimics of 2-oxoglutaric acid, such as the acylcyclohexanedionestrinexapac-ethyl and prohexadione-calcium, interfere with the late stepsof gibberellin biosynthesis. Other plant growth regulators, such asmefluidide, inhibit cell division and differentiation.

High quality, healthy turf is essential, for example, to the golfingindustry. Accordingly, there is a continued need for maintaining suchhigh quality turf even in the incidence of drought or other threateningconditions.

SUMMARY

By way of summary, the present invention includes methods of suppressingor controlling abiotic stress on warm-season turfgrass which comprisesapplying to the turfgrass, the locus thereof or the seeds thereof, aturf quality enhancing amount of a composition comprisingacibenzolar-s-methyl and, optionally, trinexapac-ethyl.

The methods of the present invention are suitable for suppression orcontrolling abiotic stress and further improving the quality of theturfgrass to which it is applied. Accordingly, the methods of thepresent invention are useful both to (1) manage abiotic stress bysuppressing or controlling the abiotic stress and (2) enhance thequality of the turfgrass to which it is applied. The method of theinvention enhances the ability of end-users such as lawn care operators,golf course technicians and the like to manage, suppress or controlturfgrass abiotic stress issues without undue phytotoxicity.

The above summary is intended to summarize certain embodiments of thepresent disclosure. Systems, methods and compositions will be set forthin more detail, along with examples demonstrating efficacy, in thefigures and detailed description below. It will be apparent, however,that the detailed description is not intended to limit the presentinvention, the scope of which should be properly determined by theappended claims.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a graphical representation of the results provided fromExample 1 relating to clipping yield;

FIG. 2 is a graphical representation of the results provided fromExample 1 relating to soil volumetric water content;

FIG. 3 is a graphical representation of the results provided fromExample 1 relating to green cover;

FIG. 4 is a graphical representation of the results provided fromExample 2 relating to clipping yield;

FIG. 5 is a graphical representation of the results provided fromExample 2 relating to mean visual turf quality;

FIG. 6 is a graphical representation of the results provided fromExample 2 relating to mean color rating;

FIG. 7 is a graphical representation of the results provided fromExample 2 relating to mean visual turf quality;

FIG. 8 is a graphical representation of the results provided fromExample 2 relating to mean color rating;

FIG. 9 is a graphical representation of the results provided fromExample 3 relating to evapotranspiration vs. vapor pressure deficit;

FIG. 10 is a graphical representation of the results provided fromExample 3 relating to evapotranspiration vs. vapor pressure deficit;

FIG. 11 is a graphical representation of the results provided fromExample 3 relating to evapotranspiration vs. vapor pressure deficit; and

FIG. 12 is a graphical representation of the results provided fromExample 3 relating to evapotranspiration vs. vapor pressure deficit.

DETAILED DESCRIPTION

As indicated above, the present invention includes methods ofsuppressing or controlling abiotic stress on warm-season turfgrass whichcomprises applying to the turfgrass, the locus thereof or the seedsthereof, a turf quality enhancing amount of a composition comprisingacibenzolar-s-methyl (hereafter “ASM”) and, optionally, trinexapac-ethyl(hereafter “TXP”).

According to the invention, by “turfgrass” there is understood an annualor perennial Gramineae. Said gramineae preferably belongs to one or moreof the genera Agropyron, Agrostis, Axonopus, Bromus, Buchloë, Cynodon,Eremochloa, Festuca, Lolium, Paspulum, Pennisetum, Phleum, Poa,Stenotaphrum or Zoysia. In some embodiments, said gramineae belongs toone or more of the genera Agrostis, Buchloë, Cynodon, Eremochloa,Festuca, Lolium, Paspulum, Pennisetum, Poa, Stenotaphrum or Zoysia.

The methods of the present invention may be useful, in some embodiments,on warm season turfgrasses. Some warm season turfgrasses that may beuseful include, but are not limited to, Bermudagrasses (Cynodon L. C.Rich), Zoysiagrasses (Zoysia Willd.), St. Augustinegrass (Stenotaphrumsecundatum (Walt.) Kuntze), Centipedegrass (Eremochloa ophiuroides(Munro.) Hack.), Carpetgrass (Axonopus Beauv.), Bahiagrass (Paspalumnotatum Flugge.), Kikuyugrass (Pennisetum clandestinum Hochst. exChiov.), Buffalograss (Buchloe dactyloides (Nutt.) Engelm.) and Seashorepaspalum (Paspalum vaginatum swartz).

According to the invention, the “locus” of a turf can relate to soil orto a substrate. An example for such a locus is a golf course, on whichturfgrass is managed. According to the present invention the term “soil”means natural soil, which is typically present on a land area, such assoil being present on a golf course, or means soil, that has beenmodified, such as soil being granulated and/or treated withagrochemicals, such as for example fertilizers. An example of granulatedand/or treated soil is disclosed in U.S. Pat. No. 5,265,372.

According to the invention, the term “substrate” means a medium for thegrowth of turfgrass and the like, suited for application to a variety ofexisting ground structures. Typically, such mediums are soil-freemixtures that include sufficient proportions of ingredients ofelastomeric granules, suitable binding emulsion, mineral aggregate,filler and controlled release plant nutrient particles, so that whenlaid and cured, said mixture produces a water permeable, resilientsubstrate having air pockets through which a root system of turfgrasscan penetrate. Turfgrass growing on said substrate can form a turf,which can be applied to non-porous surfaces, such as for example roofsof buildings, terraces and other hard surface areas, or to poroussurfaces, such as for example football fields or golf courses. Examplesof such substrates are described in WO 2005/002323. Elastomeric granulescan be, for example, granules of rubber, granules of recycled vehicletyre rubber or mixtures thereof.

The methods of the present invention may be applicable to any type ofabiotic stress that turfgrass may experience during its growth. Forexample, in some embodiments, the methods of the present invention areapplicable when the abiotic stress experienced by a plant during itsgrowth is one or more of drought, flood, excessive temperature, lowtemperature, frost, excess sunlight, insufficient sunlight, wind,inadequate soil nutrients, excessive soil salinity, air pollution, soilpollution or water pollution. In additional embodiments, the methods ofthe present invention are applicable when the abiotic stress experiencedare one or more of drought, excessive temperature or salinity.

As further indicated above, the present methods may also be utilized toimprove the quality of the turfgrass to which it is applied. As usedherein the phrase “quality” of turfgrass is meant to include visualquality of turfgrass and functional quality of turfgrass.

“Visual quality” of turfgrass relates to the visual appearance, such asdensity (the number of aerial shoots per unit area), uniformity (forexample uniformity of texture, e.g. width of the leaf blades, which canbe fine-textured as for example in red fescue or coarse-textured as forexample in tall fescue), colour or smoothness (which affects for examplethe playability of a golf course).

“Functional quality” of turfgrass relates to, for example, rigidity(resistance of the turfgrass leaves to compression and is related to thewear resistance of a turf), elasticity (tendency of the turfgrass leavesto spring back once a compressing force is removed), resiliency(capacity of a turf to absorb a shock without altering its surfacecharacteristics), ball roll (average distance a ball travels upon beingreleased to a turf surface), yield (measure of clippings removed withmowing), verdure (measure of amount of aerial shoots remaining aftermowing), rooting (amount of root growth evident at any one time duringthe growing season) and recuperative capacity (capacity of turfgrassesto recover from damage caused by disease organism, insects, traffic andthe like).

An improvement in the quality of turfgrass provided by the presentmethods may relate to one of the mentioned visual or functional qualitycharacteristics or to any combination of these quality characteristics.According to the present invention, an “improvement” is a measurable ornoticeable increase in a given turfgrass quality characteristic over thesame turfgrass quality characteristic produced under the sameconditions, but without the application of the subject method. Animprovement in the quality characteristics of turfgrass is, for example,a greener or more pleasant, leaf colour of the turf.

As indicated above, the methods of the present invention may be usefulin suppressing abiotic stress or stresses in warm-season turfgrasses. Insome embodiments of the present invention, ASM may be applied at a rateof between about 4 and about 200 g/ha per application. In furtherembodiments of the invention, ASM may be applied at a rate between about12 and about 150 g/ha per application, or between 12 and about 96 g/haper application, or between about 12 and about 64 g/ha per application,or between about 12 and about 32 g/ha per application, or between about32 and about 140 g/ha per application, or between about 32 and about 96g/ha per application, or between about 32 and about 64 g/ha perapplication, or between about 64 and about 140 g/ha per application, orbetween about 64 and about 96 g/ha per application, or between about 96and about 140 g/ha per application. Utilizing such rates per applicationon warm-season turfgrasses, the Inventors have found that abioticstresses of the warm-season turfgrasses may be lessened while alsoavoiding unacceptable phytotoxicity to the grasses. In contrast,utilizing such rates on cool-season grasses has been found to provideunacceptable levels of phytotoxicity, which leads to grasses that do notreach the standards of appropriate visual and playable quality by golfsuperintendents.

If TXP is utilized in the methods of the present invention, it may beapplied at a rate of between about 15 and about 1000 g/ha perapplication, or at a rate between about 24 and about 140 g/ha perapplication, or between about 64 and about 140 g/ha per application, orbetween about 96 and about 140 g/ha per application. The user'sspecifications, including type of warm-season turfgrass may influencethe type of rate utilized.

In addition, the rates utilized of the ASM and other, optionally-appliedactive components may vary based on the type of grass or use of grass towhich they are being applied. For example, on a golf course, fairwaysand greens are typically sprayed with different rates of activeingredients. Accordingly, compositions utilized in the methods of thepresent invention may be applied in any of the rates expressed above forASM or TXP.

If ASM is to be applied with TXP or another active ingredient, asdiscussed further below, the ratios of ASM to the other activeingredient may be between about 2000:1 to about 1:2000. In otherembodiments, the ratios of ASM to other active ingredients may bebetween about 1000:1 and about 1:1000, or between about 500:1 and about1:500, or between about 100:1 and about 1:100. In addition, in furtherembodiments of the invention, the ratio between ASM and an additionalactive ingredient may be between 5:1 and about 1:5, or between about 2:1to about 1:2, or 1:1.

The amount of ASM and, optionally, TXP or other active ingredient to beapplied and the number of re-treatments in the practice of a methodaccording to the invention will depend on various factors, such as thesubject of the treatment, such as, plants, turfgrass locus or seeds; thetype of treatment, such as, for example spraying, spreading or seeddressing; the purpose of the treatment, such as, for example suppressionor control abiotic stress; the type of abiotic stress to be controlled;enhancing turf quality, the application time; environmental conditions,the number of re-treatment intervals desired or the turfgrass species.

According to the present invention the term “applied” means eithersimultaneously or sequentially. Accordingly, if ASM is applied with asecond active ingredient, including TXP, the actives may be appliedeither simultaneously or sequentially. If administered sequentially, thecomponents may be administered in any order in a suitable timescale, forexample, with no longer than 24 hours between the time of administeringthe first component and the time of administering the last component.Suitably, all the components may be administered within a timescale of afew hours, such as one hour. If the components are administeredsimultaneously, they may be administered separately or as a tank mix oras a pre-formulated mixture of all the components or as a pre-formulatedmixture of some of the components tank mixed with the remainingcomponents.

ASM and, optionally, trinexapac-ethyl containing compositions used inthe method of the invention can be prepared on site by the end-usershortly before application to the turfgrass, the locus thereof or seedsthereof by mixing in aqueous solution an ASM containing composition anda separate active ingredient containing composition, one or moreoptional additional active ingredient containing composition(s) and,optionally, one or more suitable surfactant(s) or adjuvant(s). Suchcompositions are typically referred to as “tank-mix” compositions.

Alternatively, the compositions used in the method of the invention maybe provided to the end-user already formulated, either at the desireddilution for application (“ready to use” compositions) or requiringdilution, dispersion, or dissolution in water by the end-user(“concentrate” compositions). In some embodiments, the preformulatedconcentrates may be liquids or particulate solids. In another aspect ofthe methods of the invention, ASM can be applied to the turfgrass or thelocus thereof using turfgrass seed as a carrier.

The compositions used in the methods according to the invention maycomprise one or more additional active ingredients, such as a fungicide,insecticide, herbicide or additional growth regulator. An example wouldbe a composition that comprises a fungicide. Any suitable fungicide orherbicide may be used in the composition, for example to provide controlof pests, to overcome problems and delay the onset of resistance, or toprovide improved efficacy though an additive or synergistic effect ofthe active ingredients. Turf wetting agents may also be used inconjunction with the method of the invention. Suitable examples includeRevolution® or Radiance® (Aquatrols); and Qualibra™ (Syngenta).

As indicated above, the methods of the invention for suppressing orcontrolling abiotic stress on turfgrass with ASM and, optionally, TXPcontemplate one or more additional active ingredients being appliedincluding, but not limited to, azoxystrobin; additional plant growthregulators such as uniconazole, and paclobutrazol; neonicotinoids suchas thiamethoxam and imidacloprid; bisamides such as cyantraniliprole andchlorantraniliprole; fluazinam; propiconazole, difenoconazole,cypraconazole; fludioxonil; mefenoxam; cyprodinil; thiophanate methyl;iprodione; triadimefon; propamocarb; fosetyl-al; flurprimidol;flutalonil; pyraclostrobin; boscalid; vinclozolin; trifloxystrobin;myclobutanil; fenarimol; SDHI fungicides such as penthiopyrad,isopyrazam, fluxapyroxad, fluopyram and solatenol; fluoxastrobin;phophonic acid derivatives such as phosphonic acid, monopotassium salt;abamectin; cis-jasmone; abamectin iron chelate mixtures; and lambdacyhalothrin.

In additional embodiments, the methods of the invention contemplate anabiotic stress reducing effective amount of a composition comprising ASMand, optionally, TXP as noted above with fungicides having protectantmodes of fungicidal action being applied to the turfgrass or to thelocus of the turfgrass. Suitable fungicides with protectant modes ofaction include, for example, fluazinam and mancozeb.

The compositions used in the methods of the invention may be employed inany conventional form, for example in the form of a twin pack, a powderfor dry seed treatment (DS), an emulsion for seed treatment (ES), aflowable concentrate for seed treatment (FS), a solution for seedtreatment (LS), a water dispersible powder for seed treatment (WS), acapsule suspension for seed treatment (CF), a gel for seed treatment(GF), an emulsion concentrate (EC), a suspension concentrate (SC), asuspo-emulsion (SE), a capsule suspension (CS), a water dispersiblegranule (WG), an emulsifiable granule (EG), an emulsion, water in oil(EO), an emulsion, oil in water (EW), a micro-emulsion (ME), an oildispersion (OD), an oil miscible flowable (OF), an oil miscible liquid(OL), a soluble concentrate (SL), an ultra-low volume suspension (SU),an ultra-low volume liquid (UL), a technical concentrate (TK), adispersible concentrate (DC), a wettable powder (WP) or any technicallyfeasible formulation in combination with agriculturally acceptableadjuvants.

Said compositions used according to the methods of invention may beproduced in conventional manner, e.g. by mixing the ASM with at leastone appropriate formulation adjuvant.

The term “formulation adjuvant” according to the invention denotes anatural or synthetic, organic or inorganic material with which thecompound of formula I is combined in order to facilitate its applicationto turf. This adjuvant is hence generally inert, and it must beagriculturally acceptable, in particular to turf.

The formulation adjuvant can be a carrier or a surfactant. Incompositions according to the invention more than one adjuvant can bepresent, in such embodiments more than one carrier and/or more than onesurfactant can be present, a non-limiting example would be one carrierand two surfactants.

The “carrier” can be a liquid carrier (water, alcohols, ketones,petroleum fractions, aromatic or paraffinic hydrocarbons, chlorinatedhydrocarbons, liquefied gases, and the like) or a solid carrier.

Suitable liquid carriers are, but are not restricted to: aromatichydrocarbons, in particular the fractions C₈ to C₁₂, such as xylenemixtures or substituted naphthalenes, phthalic esters such as dibutyl ordioctyl phthalate, dipropylene glycol dibenzoate, aliphatic hydrocarbonssuch as cyclohexane or paraffins, alcohols and glycols as well as theirethers, esters and diesters, such as ethylene glycol monomethyl ether,ketones such as cyclohexanone, strongly polar solvents such as, but notrestricted to, N-methyl-2-pyrrolidone, dimethyl sulfoxide ordimethylformamide, and, if appropriate, epoxidized vegetable oils orsoybean oil; or water.

Suitable solid carriers are, but are not restricted to: aluminiumsilicate, urea, sodium sulphate, talc, calcium sulphate or potassiumsulphate and seed.

According to the invention a single carrier or a mixture of two or morecarriers may be present in the composition(s) used in the methodsaccording to the invention.

“Surfactants” are non-ionic, cationic, amphoteric and/or anionicsurfactants having 35 good emulsifying, dispersing and wettingproperties. According to the invention a single surfactant or a mixtureof two or more surfactants may be present. The surfactants customarilyemployed in formulation technology are described, inter alia, in thefollowing publications: “McCutcheon's Detergents and EmulsifiersAnnual”, MC Publishing Corp., Glen Rock, N.J., 1988 and M. and J. Ash,“Encyclopedia of Surfactants”, Vol. I-Ill, Chemical Publishing Co., NewYork, 1980-1981.

Among the surfactants there may be mentioned, e.g., polyacrylic acidsalts, lignosulphonic acid salts, phenolsulphonic or (mono- ordi-alkyl)naphthalenesulphonic acid salts, laurylsulfate salts,polycondensates of ethylene oxide with lignosulphonic acid salts,polycondensates of ethylene oxide with fatty alcohols or with fattyacids or with fatty amines, substituted phenols (in particularalkylphenols or arylphenols such as mono- and di-(polyoxyalkylenealkylphenol) phosphates, polyoxyalkylene alkylphenol carboxylates orpolyoxyalkylene alkylphenol sulfates), salts of sulphosuccinic acidesters, taurine derivatives (in particular alkyltaurides),polycondensates of ethylene oxide with phosphated tristyrylphenols andpolycondensates of ethylene oxide with phosphoric esters of alcohols orphenols.

A seed dressing formulation is applied in a manner known per se to theseeds employing the compositions according to the invention and adiluent in suitable seed dressing formulation form, e.g. as an aqueoussuspension or in a dry powder form having good adherence to the seeds.Such seed dressing formulations are known in the art. Seed dressingformulations may contain the active ingredients in encapsulated form,e.g. as controlled release capsules or microcapsules.

The compositions used in the methods according to the invention maycomprise one or more formulation additives, such as, but not limited to,biocides, anti-freeze, stickers, thickeners and compounds that provideadjuvancy effects including, for example, one or more phthalocyanines orcopper phthalocyanines including pigment green such as pigment green 7;phthalocyanine green g; or pigment green 42.

In general, when phthalocyanines or copper phthalocyanines are presentin compositions used in the methods according to the invention, the rateof application to the turfgrass or to the locus of the turfgrass is from0.001 to 10 kilograms of phthalocyanine per hectare (kg/ha), suitablyfrom about 0.01 to about 2 kg/ha, more suitably from about 0.1 to about1 kg/ha, most suitably from about 0.2 to about 0.8 kg/ha.

In general, the compositions according to the invention include from0.01 to 90% by weight of a compound of ASM, from 0.01 to 90% by weightof a compound of TXP or other active ingredient, and from 0 to 20%surfactant and from 10 to 99.99% carrier.

Application to Turfgrass:

The methods according to the invention can be practiced by treating theturfgrass with an effective amount of ASM and, optionally, TXP accordingto the invention. Within said embodiment of the invention, the ASM and,optionally, TXP is suitably applied to the turfgrass by spraying orspreading. Treatment of turfgrass in accordance with the methods of theinvention may be performed by lawn care operators or golf coursetechnicians using known techniques.

In one embodiment of the inventive methods, to maintain high quality,healthy turfgrass on the intended surface area of ground, such as forexample, a golf course, a sports field, a park area or a home lawn, andto protect said turfgrass against abiotic stress, ASM and, optionally,TXP are applied to the turfgrass once or more than once duringmaintenance of the turfgrass.

In some embodiments of the present inventions, the method of suppressingor controlling abiotic stress comprises a treatment regime, where ASMand, optionally, TXP are applied between about 2 and about 8 times, orbetween about 3 and 4 times, to the turfgrass or to the locus thereof atintervals of from 5 to 25 days, up to the cumulative maximum labeledrate for ASM and, optionally, TXP per turf growing season for thespecific turfgrass locus being treated according to existingrestrictions.

In a more specific embodiment, in the practice of the methods of theinvention, ASM and, optionally, TXP are applied between about 1 and 20times, or between about 2 and 18 times, or between about 3 and 15 times,or between about 4 and 12 times, or between about 2 and about 8 times,or between about 3 and 4 times to the turfgrass or the locus thereof atintervals of from 7 to 21 days; or from 7 to 14 days, or from 14 to 21days up to the cumulative maximum labeled rate for ASM and, optionally,TXP for the turfgrass locus being treated.

In another aspect, suppression or control of abiotic stress is achievedby re-applying a turf quality enhancing amount of the combination of ASMand, optionally, TXP, suitably at the foregoing rates and weight ratios,at intervals of from 5 to 21 days, suitably from 7 to 14 days, duringthe turf growing season.

In a particular embodiment, 2 to 8 applications of ASM and, optionally,TXP are applied at 21 day intervals, pre-disease or under low tomoderate disease pressure during the turf growing season.

In another embodiment, 10 to 15 applications of ASM and, optionally TXP,are applied at 14 day intervals, pre-disease.

Application to the Locus of the Turfgrass:

The compositions used in the method according to the invention can beapplied to the turfgrass by treating the locus of the turfgrass with acomposition comprising ASM and, optionally, TXP according to theinvention.

Application of ASM and, optionally, TXP compositions used in the methodof the invention to a locus covers liquid (sprayable) or granular(active ingredient (a.i.) on inert and a.i. on fertilizer) (spreadable)applications as well.

For example, in the practice of method of the invention compositionscomprising ASM and, optionally, TXP can be applied to the soil before orafter the seeds of the turfgrass are sown or placed into the soil; orsuch compositions are applied according to the method of invention to asubstrate for the growth of turfgrass before or after the seeds of theturfgrass are placed into the substrate; or the compositions appliedaccording to the method of the invention can be applied to the soilbefore turfgrass grown on a substrate are placed on top of the soiltogether with the substrate.

In one embodiment, according to the method of the invention compositionscomprising ASM and, optionally, TXP are applied to the turfgrass as asprayable liquid formulation. In another embodiment, such compositionsare applied to the turfgrass as a granular formulation. Suitablegranules include inert and fertilizer granules. The active ingredientmay be dispersed throughout, impregnated into, or coated on the surfaceof the granules.

Application to the Seeds of the Turfgrass:

The method according to the invention also can be practiced by applyingthe compositions containing ASM and, optionally, TXP to the seeds of theturfgrass by treating the seeds with such a composition. When the methodaccording to the invention involves using ASM and, optionally, TXPcompositions for treating seed, rates of 0.001 to 50 g of the compoundmixture per kg of seed, suitably from 0.01 to 10 g per kg of seed, aregenerally sufficient. In one embodiment, an amount of seed used as acarrier for ASM and, optionally, TXP can be applied to the soil orsubstrate to deliver a suitable amount of the active ingredient.

BIOLOGICAL EXAMPLES

The following Examples and the results provided in the Figuresillustrate the effectiveness of the present invention

Example 1

Four applications of treatments were made on 21 day intervals throughthe season on Tifsport hybrid bermudagrass maintained at 1.27 cm. Fourreplications of each treatment were completed. The treatments included:(1) an untreated check; (2) TXP applied at a rate of 96 g/ha; (3) TXPapplied at a rate of 96 g/ha and ASM applied at a rate of 32 g/ha; (4)TXP applied at a rate of 96 g/ha and ASM applied at a rate of 64 g/ha;(5) TXP applied at a rate of 96 g/ha and ASM applied at a rate of 96g/ha; (6) ASM applied alone at a rate of 32 g/ha; (7) ASM applied aloneat a rate of 64 g/ha; and (8) ASM applied alone at a rate of 96 g/ha.The plots were exposed to a dry down cycle by withholding irrigationthree days after the second TXP treatment, where irrigation was withheldfor up to 40 days and resumed to avoid turf loss. Clippings werecollected two times per week and turf quality was visually rated weeklyduring the drought cycle. Soil volumetric water content was measuredevery two days during the dry-down cycle using a Spectrum Field ScoutTDR probe.

The results of the testing, including clipping yields (FIG. 1), soilvolumetric water content (FIG. 2), and greening rating (FIG. 3) areshown in the Figures.

Example 2

Three applications of treatments were made on 21 day intervals throughthe season (June 21, July 11, and August 4) on Tifsport hybridbermudagrass maintained at 0.500 inches. The treatments included: (1) anuntreated check; (2) TXP applied at a rate of 96 g/ha; (3) TXP appliedat a rate of 96 g/ha and ASM applied at a rate of 32 g/ha; (4) TXPapplied at a rate of 96 g/ha and ASM applied at a rate of 64 g/ha; (5)TXP applied at a rate of 96 g/ha and ASM applied at a rate of 96 g/ha;(6) ASM applied alone at a rate of 32 g/ha; (7) ASM applied alone at arate of 64 g/ha; and (8) ASM applied alone at a rate of 96 g/ha. Theplots were exposed to a dry down cycle by withholding irrigationfollowing applications 2 and 3. Clipping collections were performed justprior to the 2^(nd) application and continued twice per week through thecompletion of the trial.

The results of the testing, including clipping yields (FIG. 4), meanvisual turf quality (FIGS. 5 and 7), and mean color rating (FIGS. 6 and8) are shown in the Figures.

Example 3

Fifteen applications of treatments were made on 14 day intervals throughthe season (starting in May and extending through October) on UltradwarfBermudagrass on golf course greens. The treatments included (1) aControl that included products with the active ingredients ofchlorothalonil, fluazinam and azoxystrobin and no ASM and (2) atreatment that included products with the same active ingredients andrates of the Control treatment, but also included 30.8 g/ha rate of ASMfor each spray.

Turfgrass Samples from the treated plots were taken from a) the ASMtreated plots as well b) the Control plots without acibenzolar-s-methylat two different timings—July 2015 and September 2015. All samples weretested for evapotranspiration at various vapor pressure deficits (VPD)in the growth chamber. The results of the testing are illustrated inFIGS. 9-12. The results indicate that as VPD increases, those plotswithout ASM form a natural linear regression. However the ASM treatedturfgrass is able to decrease its rate of transpiration causing anoverall reduced evapotranspiration as VPD increases, forming anon-linear regression. ASM allows the turfgrass to hold on to more wateras temperatures increase and results in improved turf quality even inthe face of increased drought or heat stress. In addition, the rates ofASM did not result in any unacceptable phytotoxicity to the turfgrass.

Although the invention has been described with reference to preferredembodiments and examples thereof, the scope of the present invention isnot limited only to those described embodiments. As will be apparent topersons skilled in the art, modifications and adaptations to theabove-described invention can be made without departing from the spiritand scope of the invention, which is defined and circumscribed by theappended claims. All publications cited herein are hereby incorporatedby reference in their entirety for all purposes to the same extent as ifeach individual publication were specifically and individually indicatedto be so incorporated by reference.

1. A method for controlling or suppressing abiotic stress in warm-seasonturfgrass, comprising: applying to the turfgrass or to the locus of theturfgrass, a turf quality improving amount of a composition comprisingacibenzolar-S-methyl at a rate between about 32 and 96 g/ha.
 2. Themethod of claim 1, wherein the warm season turfgrass is Bermudagrass. 3.The method of claim 1, wherein the warm-season turfgrass is Zoysiagrass.4. The method of claim 1, wherein the composition further comprisesadjuvants, solvents, carrier, surfactants or extenders.
 5. The method ofclaim 4, wherein the composition comprises from 0.01 to 90% by weight ofthe acibenzolar-S-methyl, from 10 to 99.99% of a carrier and from 0 to20% of a surfactant.
 6. The method of claim 1, whereinacibenzolar-S-methyl is applied at the rate of from 32 to 64 g/ha. 7.The method of claim 1, wherein acibenzolar-S-methyl is applied betweenabout two and 18 times, at intervals of from 5 to 21 days during theturf growing season.
 8. The method of claim 1, whereinacibenzolar-S-methyl is applied between about three and four times, atintervals of from 14 to 21 days during the turf growing season.
 9. Themethod of claim 1, wherein the abiotic stress relates to droughtconditions.
 10. The method of claim 1, wherein the method furthercomprising applying trinexapac-ethyl to said warm-season turfgrass.